Hi all,

I have targeted metabolomics data which have been normalized using the loess method. I want to ask if further data pre-processing is needed namely standardization (divide with standard deviation of each metabolite) or log transformation of each metabolite because I have huge values. The distributions look slightly skewed for some metabolites.

I want to run a network analysis using WGCNA (weighted gene co-expression network analysis) which is based on computing pairwise correlations. Thus I'm wondering if it is important to standardize or log-transform the data or apply another pre-processing approach before starting the analysis of the metabolites.

Thanks in advance for your help.

Best, Jane